New drug prevents bacteria from acquiring antibiotic resistance genes

According to a new study, scientists have developed a drug that prevents bacteria from acquiring the genes needed to develop antibiotic resistance.

Bacteria evolve resistance to antibiotics by acquiring DNA fragments from their environs and incorporating them into their genomes. This ability is referred to as "competence."

Through a series of observations and lab tests, scientists were able to identify the chain of events that allow bacteria cells to become competent. Once they identified what competence looked like, scientists were able to identify drugs that interfered with the process.

"We collaborated with scientists from Heidelberg, who developed a high-throughput assay to simultaneously test cells for competence and growth," lead study author Arnau Domenech, postdoctoral researcher at the University of Groningen in the Netherlands, said in a news release. "In this assay, 1,366 approved drugs were screened. It turned out that 46 of them blocked the induction of competence, without negatively affecting growth."

Cells can become motivated to evolve defenses against drugs if the drugs cause growth stress. Because the 46 drugs don't negatively impact cell growth, cells of the bacterium Streptococcus pneumoniae didn't develop resistance.

The drugs that prevented competence and didn't inhibit cell growth belonged to two different categories: drugs affecting ion homeostasis and antipsychotics. Further investigation showed that all of the drugs block competence by disrupting the proton-motive force, the electrochemical gradient that propels protons cellular membranes and fuels a variety of cellular processes.

"The result is that the cells fail to secrete a peptide called CSP," said Domenech.

When enough cells secrete CSP, competence genes get activated -- a process known as quorum sensing. By disrupting the proton-motive force, the 46 drugs prevented CSP secretion from crossing the necessary threshold and turning on the competence genes.

"In the lab, we observed that our competence-blocking drugs could prevent the transfer of antibiotic resistance genes to susceptible strains of Streptococcus pneumoniae and we obtained the same results in cultures of human lung epithelial cells," Domenech said.

Scientists got the same promising results when they repeated the lab tests using a mouse model. The research team published the results of their tests this week in the journal Cell Host and Microbe.

Because the proton-motive force is necessary for some vital functions in humans, scientists aren't certain whether drugs are safe for humans. Further testing is needed to determine whether these anti-evolution drugs can help prevent antibiotic resistance in humans without harming cell growth.

"Nevertheless, we discovered a general pathway that we can block to prevent the spread of antibiotic resistance," said Domenech.

Related Links
Space Medicine Technology and Systems

Tweet

Thanks for being there;
We need your help. The SpaceDaily news network continues to grow but revenues have never been harder to maintain.

With the rise of Ad Blockers, and Facebook - our traditional revenue sources via quality network advertising continues to decline. And unlike so many other news sites, we don't have a paywall - with those annoying usernames and passwords.

Our news coverage takes time and effort to publish 365 days a year.

If you find our news sites informative and useful then please consider becoming a regular supporter or for now make a one off contribution.

SpaceDaily Monthly Supporter
$5+ Billed Monthly

paypal only

SpaceDaily Contributor
$5 Billed Once

credit card or paypal

UCLA engineers develop miniaturized 'warehouse robots' for biotechnology applications
Los Angeles CA (SPX) Feb 27, 2020
UCLA engineers have developed minuscule warehouse logistics robots that could help expedite and automate medical diagnostic technologies and other applications that move and manipulate tiny drops of fluid. The study was published in Science Robotics. The robots are disc-shaped magnets about 2 millimeters in diameter, designed to work together to move and manipulate droplets of blood or other fluids, with precision. For example, the robots can cleave one large droplet of fluid into smaller drops th ... read more